AI Startups Funding: 33 US Firms Raise $100M+ in 2025

AI startups funding in the US has reached unprecedented levels in 2025, with 33 firms securing mega-rounds of $100 million or more. This year’s capital influx underscores robust investor confidence and highlights venture capital giants such as Andreessen Horowitz, Kleiner Perkins and Sequoia Capital driving these rounds. Key sectors benefiting include healthcare AI, infrastructure and hardware, research labs, generative media and legal tech. Notable deals range from OpenAI’s record $40 billion round valuing the firm at $300 billion to emerging labs like Thinking Machines Lab raising $2 billion at a $12 billion valuation. The breadth of funding—from Series A through Series E—demonstrates AI startups funding diversity and the pervasive appeal of artificial intelligence across industries. While these mega-rounds signal rapid innovation, they also raise questions about valuation sustainability, regulatory scrutiny and talent competition. For crypto traders, the trend highlights potential partnerships between AI and blockchain, but AI startups funding remains largely distinct from direct token issuance or DeFi projects.